Biological reconstruction with frozen recycled autograft applies the bones with tumor into minus -196 degrees Celsius liquid nitrogen for 20 minutes to ensure that the central temperature of the tumor reaches the lowest temperature. Subsequently, the bones with the tumor were slowly thawed at room temperature for about 15 minutes. In this rapid freezing and slow thawing process, water molecules in cells can form large chunks of ice crystals and destroy tumor cells.

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Reconstruction with Recycled Autograft - Preserving the Inherited Bones